MS. SUCHI
Mazandaran University of Medical Sciences,Sari Iran
Title: Assessment of Triglyceride to High-density Lipoprotein Ratio as an Indicator of Coronary Artery Disease
Biography
Biography: MS. SUCHI
Abstract
Background and Aim : Parkinson’s disease is the second most common neurodegenerative disorderwhich affecting the senile population with manifestation of motor disability and cognitiveimpairment.Sambucus ebulus is a plant with traditional uses which might confer neuroprotective effectsmost probably according to its anti-oxidative stress activity but there are no studies around its role inParkinson diseases so far. Hence, this study has aimed to investigate the neuroprotective effect of totalextract of fruits and aerial parts of Sambucus ebulus in a neurotoxin-induced model of Parkinson.
Methods : In vitro model of Parkinson disease has generated by exposing SH-SY5Y neuroblastoma cellsto neurotoxin: 6-hydroxydopamine (6-OHDA) 100μM/well.Total extract of fruits and aerial parts of Sambucus ebulus extracted by tow solvents of methanol and ethyl- acetate by maceration method.Cytotprotective effect of methanol and ethyl acetate extracts in five concentrations on cell viability byusing MTT assay. Apoptotic assay was done with route of Annexin V-propidium iodide method by flowcytometry
Results : According to MTT assay analysis,both methanol and ethyl acetate extracts have shown protective effect against 6-OHDA induced cytotoxicity in SH-SY5Y neuroblastoma cells especially atconcentrations of 30 and 60 μg/ml P<0.05 but apoptetic analysis has shown at IC50 Conct, onlymethanolic extract of the heeb had anti-apoptic effect P<0.05.
Conclusion: We can introduce aerial parts of sumbacus ebulus extract as a cytoprotective co-trearmentin Parkinson disease but compelementary studies especially in in vivo and clinical trials are necessary.
Keywords: Sambucus ebulus, Apoptosis, SH-SY5Y, 6-OHDA, Neurodegenerative disorder, Parkinson’sdisease, Neuroprotection