Wuwei Ye
Director of Biotechnology Research, China
Title: Innovation of cotton Germplasm of Draught and Salinity tolerance
Biography
Biography: Wuwei Ye
Abstract
Cotton, a kind of crops with strong drought- and salt-resistance, is important to explore excellent drought- or salt-tolerant genes and improve the level of drought- or salt-tolerance on cotton. The study was performed with Gossypium hirsutum cv ZhongH177 and Zhong9835 as the material, 12 genes of drought- or salinity-resistance were isolated from the mitochondria, which plays an essential role in drought- and salt-tolerance of plants. Based on 0.4% salt stress method, we obtained three salt-tolerance related gene ccmC, rps12 and nad3 from mitochondria of cotton. We cloned them from (Gossypium hirsutum L., a drought- and salt-tolerance variety). Subsequently, the analysis of biological information were used; subcellular localization of those genes were found out; based on over-expression vector, transformed into Arabidopsis thaliana by floral-dip method and transformed into cotton by gene gun transgenic technology. The over-expression vector pBI121-rps12, pBI121-ccmC and pBI121-nad3 were constructed and transferred into cotton by gene gun transgenic technology, which laid a certain foundation for further analysis salt-tolerant molecular mechanism and salt resistance germplasm innovation of cotton.