M. Taherianfard
Shiraz University, Iran
Title: Pretreatment of Hydro-Alcoholic Extract of Ginseng can modulate the analgesic effect of D2 Dopamine Receptor in Rats
Biography
Biography: M. Taherianfard
Abstract
It seems that ginseng root analgesic effects are through dopaminergic system. Therefore, the aim of present study was to evaluate the effect of pretreatment with hydro-alcoholic extract of ginseng and bromocriptine (dopamine D2 receptor agonist) and chlorpromazine (dopamine D2 receptor antagonists) on pain sensitivity in rats.
Methods: Seventy adult male rats were randomly divided into 7 groups: 1-Sham 1 (received IP injection of saline); 2- Sham 2 (received ICV injection of ACSF); 3-Exprimental 1 (received IP injection ginseng 100 mg/kg/day for 7 days); 4 & 5- Experimental 2 and 3 (received preatretment of ginseng same as group 3 and ICV injection of bromocriptine 10 and 30 ug/rat after) and 6 and 7- Expermental 4 and 5 (received preatretment of ginseng same as group 3 and ICV injection of chlorpromazine 20 and 40 ug/rat after). Pain test was performed by formalin test. Cannula unilaterally implanted into the lateral ventricle by stereotoxic method.
Results: The data showed that ginseng significantly (P<0.05) decreased pain sensitivity in both phases of the formalin test. Pretreatment of ginger and received 2 doses of bromocriptine significantly (P<0.05) decreased pain sensitivity in both phases of formalin test in comparison to both sham groups and experimental 1 group. Pretreatment of ginger and received 2 doses of chlorpromazine significantly (P<0.05) increased pain sensitivity in both phases of formalin test in comparison to both sham groups and experimental 1 group.
Conclusions: According to the present results, ginseng can modulate pain sensitivity in the formalin test through dopaminergic system.