Yoanna Yordanova Koedzhikova
Medical university of Sofia, Bulgeria
Title: Effects Of Newly Synthetized Hydrazones Of N-Pirolylcarboxylic Acid On Isolated Rat Brain Synaptosomes
Biography
Biography: Yoanna Yordanova Koedzhikova
Abstract
This study investigate the in vitro effects of newly synthesized hydrazones of N-pirolylcarboxylic acid on isolated rat brain synaptosomes. The main parameters, characterized the functional status of synaptosomes: synaptosomal viability and depletion of reduced glutathione (GSH), were measured.
Administered alone, all the compounds (at concentration 50 µM) revealed statistically significant neurotoxic effects on the synaptosomes, compared to the control (non-treated synaptosomes). With lower toxic effects were compound DI5a and DI5g. These compounds (DI5a and DI5g) were exam for possible neuroprotective effects in a model of 6-hydroxydopamine (6-OHDA)-induced oxidative stress.
The treatment of isolated rat brain synaptosomes with 6-OHDA is a reliable and commonly used in vitro model for the investigation of processes, which play role in the neurodegenerative disease, including Parkinson’s and Alzheimer’s disease. The mechanism of 6-OHDA neurotoxicity includes the formation of ROS and reactive metabolites, as a result of its metabolism in mitochondria of the neuronal cells.
In conditions of 6-OH-dopamine-induced oxidative stress (at concentration 150 μM) on isolated rat synaposomes, DI5a and DI5g (at concentration 50 μM) revealed statistically significant neuroprotective effects by preservation the synaptosomal viability (measured by MTT-test), and GSH deletion, compared to the toxic agent.