Elena Emiliyanova Smilyanova
Medical university of Sofia, Bulgeria
Title: Effects Of Newly Synthetized Derivatives Of Caffeine-8-Α-Methyl Thioglycolic Acid On The Activity Of Human Recombinant Maob Enzyme
Biography
Biography: Elena Emiliyanova Smilyanova
Abstract
New series of caffeine-8-α-methyl thioglycolic acid derivatives (at concentration 1 µM) were investigated for possible inhibiting effects on human recombinant MAOB enzyme (hMAOB).
hMAOB is responsible for the formation of the neurotoxic reactive metabolites. It’s inhibition could be one of the mechanisms for possible neuroprotection against some neurodegenerative disease, including Parkinson’s and Alzheimer’s disease
Monoamine oxidase activity assay of recombinant human MAOB was performed using a fluorometric method by Amplex UltraRed reagent. Tyramine hydrochloride was used as substrate.
The effects of the compounds were compared with Selegiline (at concentration 1 µM).
All the compounds didn’t revealed statistically significant inhibitory activity on the hMAOB enzyme.